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. 2000 Aug 15;97(17):9537–9542. doi: 10.1073/pnas.97.17.9537

Figure 1.

Figure 1

Experimental design. (a) Structure of the URA3-arg4 recombination substrates. Thick line, pBR322 sequences; hatched box, 1.2-kb HindIII URA3 fragment; gray box, 3.3-kb PstI arg4 fragment containing either arg4-nsp or arg4-bgl; thin lines, flanking genomic sequences; horizontal arrows, direction of ARG4 transcription; vertical arrows, meiosis-induced DSBs seen in all inserts (36). (b) Ectopic recombination was measured between pairs of arg4 inserts (open arrows, indicating insert orientation) on chromosomes III, V, and VIII. Ectopic recombination was measured in three different diploid types: (i) nonhybrid diploids with only S. cerevisiae chromosomes (solid lines), (ii) diploids with a single homoeologous S. carlsbergensis chromosome V, and (iii) diploids with homoeologous copies of S. carlsbergensis chromosomes III and V. Hatched lines on S. carlsbergensis chromosomes indicate regions of divergence from S. cerevisiae.