Table 2.
Insert location | ndj1 | NDJ1† | ndj1/NDJ1 |
---|---|---|---|
Allelic recombination, f(ARG4) × 103* | |||
LEU2 | 19 | 19 | 1.0 |
URA3 | 8.1 | 5.7 | 1.4 |
CHA1 | 5.0 | 4.7 | 1.1 |
PHO11 | 3.0 | 4.8 | 0.6 |
PHO12 | 1.3 | 1.6 | 0.8 |
MAT | 7.4 | 9.4 | 0.8 |
Mean | 0.9 ± 0.3 | ||
Ectopic recombination, Eab‡ | |||
On heterologous chromosomes | |||
MAT × URA3 | 0.29 | 0.12 | 2.4 |
LEU2 × URA3 | 0.29 | 0.13 | 2.2 |
PHO11 × PHO12 | 0.51 | 0.24 | 2.1 |
PHO11 × CHA1 | 0.59 | 0.27 | 2.2 |
PHO12 × CHA1 | 0.57 | 0.28 | 2.0 |
Mean | 2.2 ± 0.1 | ||
On homologs | |||
LEU2 × CHA1 | 0.6 | 0.56 | 1.1 |
LEU2 × MAT | 0.5 | 0.48 | 1.1 |
MAT × CHA1 | 0.62 | 0.4 | 1.6 |
Mean | 1.3 ± 0.3 |
Arg+ spores/total spores from strains with URA3-arg4 inserts at allelic locations.
Data from ref. 22.
Eab, efficiency of ectopic recombination (22). Eab for a pair of insert loci is the sum of the ectopic recombination frequencies for both marker orientations divided by the sum of the two relevant allelic recombination frequencies, corrected for loss of recombinants on lethal crossover-associated rearrangements. For allelic inserts, Eab = 1; Eab < 1 indicates that two inserts recombine less frequently than would be expected if they were at allelic positions.