Fig. 5. Sensitivity of furanone C-30-treated P.aeruginosa biofilms to tobramycin. Scanning confocal laser microscopy (SCLM) photomicrographs of P.aeruginosa PAO1 biofilms grown in the absence (left panel) or presence (right panel) of 10 µM C-30. After 3 days, the biofilms were exposed to 100 µg/ml tobramycin for 24 h. Bacterial viability was assayed by staining using the LIVE/DEAD BacLight Bacterial Viability Kit: red areas are dead bacteria, and green areas are live bacteria. The biofilms were exposed to (A) no furanone and 100 µg/ml tobramycin, (B) 10 µM C-30 and 100 µg/ml tobramycin, (C) non-treated control and (D) 10 µM C-30 and no tobramycin.