Table 3.

In vivo treatment with CBD suppresses CII-specific T helper 1 responses

Mice	[3H]Thymidine incorporation, cpm		IFN-γ production, pg/ml
	−CII	+CII	−CII	+CII
Control	3,301 (±2,540)	12,919 (±10,055)	950 (±1,397)	70,258 (±44,321)
CBD-treated	1,726 (±724)	6,574 (±3,779)*	502 (±870)	423 (±545)**

Mice were treated with CBD, 5 mg/kg i.p., or with vehicle control from onset of arthritis. They were killed at day 3, and inguinal LNCs were cultured with or without CII. After 72 h, CII-specific cell proliferation and IFN-γ release were measured. Results are the mean +/− SD of three individual mice per treatment group, each of them tested in triplicate. This experiment is representative of three reproducible experiments. In total, 10 mice per group were tested. *, not significant; **, P = 0.0027 (Mann-Whitney U).