Fig. 5. Control of IL-6 synthesis by ζPKC and RelA Ser311 phosphorylation. (A) EFs from wild-type (WT) or ζPKC-deficient mice (KO) were stimulated with different concentrations of TNF-α for 24 h, after which IL-6 production was determined by ELISA. (B) IL-6 production was also determined in EFs stably expressing WT or the S311A RelA mutant. Results are the mean ± SD of two independent experiments with incubations in duplicate.