Figure 4.

The TRAF6-interacting domains in T6BP. 293 cells were transiently transfected with expression vectors encoding Myc-epitope tagged T6BP deletion mutants and Flag-tagged TRAF6. Cell extracts were prepared and immunoprecipitated (IP) with anti-Flag monoclonal antibody (F) or control mouse IgG (C). Coprecipitating Myc-T6BP deletion mutants were detected by immunoblotting (IB) analysis with the anti-Myc polyclonal antibody (Top). The amounts of TRAF6 immunoprecipitated and the expression level of Myc-T6BP deletion mutants in total cell extracts were determined by immunoblotting with anti-Flag polyclonal antibody (Middle) and anti-Myc antibody (Lower). KD, kilodalton.