Figure 7.

IRAK is required for the IL-1-inducible TRAF6–T6BP complex formation. (A) 293.IL-1RI cells or I1A cells were stimulated with 10 ng/ml IL-1 for various times. Cell lysates were immunoprecipitated (IP) with anti-T6BP antibody and immunoblotted (IB) with anti-TRAF6 monoclonal antibody (Upper). The amounts of T6BP in the immunoprecipitates were determined by immunoblotting with anti-T6BP antibody (Lower). (B) I1A cells were transiently transfected with HA-tagged IRAK in pRK expression vector and stimulated with 10 ng/ml IL-1 for various times. Cell lysates were immunoprecipitated with anti-T6BP antibody and immunoblotted with anti-TRAF6 monoclonal antibody (Upper). The amounts of T6BP in the immunoprecipitates were determined by immunoblotting with anti-T6BP antibody (Lower).