Table I. Phenotypes of transformants obtained after the introduction of self-replicative plasmids containing different constructs of the carB gene into the wild-type strain.
Plasmid | Transformants |
Total | Silencing frequency (%) | |
---|---|---|---|---|
Albino | Bright yellow | |||
pMAT647 | 51 | 1561 | 1612 | 3.16 |
pMAT754 | 12 | 373 | 385 | 3.12 |
pMAT650 | 112 | 964 | 1076 | 10.4 |
pLEU4 | 0 | 1758 | 1758 | 0 |
Plasmid pLEU4 was used as control. The colour of the transformants was observed after 48 h in the dark, followed by 24 h under illumination with blue light.