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. 2000 Aug 1;97(17):9573–9578. doi: 10.1073/pnas.160099797

Figure 3.

Figure 3

Dex inhibits surface expression of IL-2R β and γc subunits and activation of Stat5 by other cytokines that use γc. (A) T cells were cultured with dex for 16 h at the doses indicated and the surface expression of IL-2R α (CD25), β (CD122), and γc (CD132) was assessed by flow cytometry as described (16). (B) T cells cultured with dex for 16 h were stimulated for 10 min with IL-2 (1 ng/ml), IL-15 (10 ng/ml), IL-7 (10 ng/ml), or IL-4 (10 ng/ml). (C) Akt kinase activation is blocked in dex-treated cells. Cell extracts were analyzed by sequential IB of the same membrane.