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. 2000 Aug 1;97(17):9573–9578. doi: 10.1073/pnas.160099797

Figure 5.

Figure 5

(A) Dex inhibits IL-2R β mRNA expression independent of de novo protein synthesis. T cells were cultured for 8 h with dex (10-6 M), in the presence or absence of cyclohexemide (CHX) (40 μg/ml), and mRNA levels were measured by using semiquantitative RT-PCR as described (16, 17). GAPDH, glyceraldehyde-3-phosphate dehydrogenase. (B) Dex suppresses JAK3 protein levels, and suppression is reversed by RU486. T cells cultured for 16 h with dex (10-7 M) in the presence or absence of RU486 (10-6 M). (C) RU486 blocks the inhibitory activity present in supernatants of cells cultured with dex. Supernatants from cells cultured with 10-6 M dex for 16 h were added to new cultures (50% final concentration) for 16 h in the presence or absence of RU486.