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. 2000 Aug 1;97(17):9573–9578. doi: 10.1073/pnas.160099797

Table 1.

Dex inhibits the binding of IL-2 to its receptor

Time of  culture, hr IL-2R density Negative control Specificity control
4 584 56 31
4+dex 289 31 33
16 410 38 16
16+dex 146 27 9

Measured using biotinylated IL-2 as recommended by the manufacturer (R&D Systems). Soybean trypsin inhibitor, biotinylated to the same degree as IL-2, was used as negative control for staining. Specificity of the IL-2 biotin reaction was tested by measuring the binding after preincubation with αhIL-2 Ab.