Table 1.
Dex inhibits the binding of IL-2 to its receptor
Time of culture, hr | IL-2R density | Negative control | Specificity control |
---|---|---|---|
4 | 584 | 56 | 31 |
4+dex | 289 | 31 | 33 |
16 | 410 | 38 | 16 |
16+dex | 146 | 27 | 9 |
Measured using biotinylated IL-2 as recommended by the manufacturer (R&D Systems). Soybean trypsin inhibitor, biotinylated to the same degree as IL-2, was used as negative control for staining. Specificity of the IL-2 biotin reaction was tested by measuring the binding after preincubation with αhIL-2 Ab.