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Southern blot analysis of BT4001 transconjugants from different donors. The Southern blot analysis was done on EcoRV- and HindIII-digested chromosomal DNA from six ermG-containing BT4001 transconjugants and an Emr transconjugant from WH504, BT4001ΩWH504. The blot was probed with fragment G, which contains sequences upstream of the ermG on CTnGERM1 (Fig. 2). The two arrows indicate the positions of two bands, which were more visible after longer exposure time of the Southern blot.
