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. 2003 Aug;69(8):4806–4813. doi: 10.1128/AEM.69.8.4806-4813.2003

FIG. 4.

FIG. 4.

Analysis of chicken feces. (A) Multiplex PCR amplification with SNP primers. (A) SNP multiplex PCR analysis of chicken feces. Raw fecal material (10 μl) was spotted onto an FTA filter and prepared for PCR. A primary amplicon was generated with the F1E-/R2B primer pair by conventional PCR and then used as DNA template in a subsequent nested amplification. Lane a, 100-bp DNA ladder; lane b, SNP multiplex PCR amplification of chicken feces. Arrows to the right of the gel indicate the amplicon sizes expected for the presence of nonhuman Cyclospora spp. (360 bp), C. cayetanensis (300 bp), and Eimeria spp. (173 bp). (B and C) Microscopic identification of an Eimeria spp. (B) and C. cayetanensis (C) by differential interference contrast. Magnification, ×800.