TABLE 2.
Mismatch PCR parameters for the differentiation of Cyclospora spp. and Eimeria spp.
| Species-specific primer | Primer specificity | Selective mismatch primer sequence | Approximate amplicon size (bp) | Nucleotide positions (GenBank no.) |
|---|---|---|---|---|
| CC719 | C. cayetanensis | 5′-GTAGCCTTCCGCGCTTCG-3′ | 298 | 711-728 (AF111183) |
| PDCL661 | C. cercopitheci, C. colobi, C. papionis | 5′-CTGTCGTGGTCATC*TGTCCGC-3′a | 361 | 641-661 (AF111184) |
| ESSP841 | Eimeria spp. | 5′-GTTCTATTTTGTTGGTTTCTAGGACCA-3′ | 174 | 815-841 (U40264) |
| CRP999 | Common reverse primer | 5′-CGTCTTCAAACCCCCTACTGTCG-3′ | 977-999 (AF111183) |
a
C. papionis has a substitution at the position indicated by an asterisk of G for C.