TABLE 3.

Transformation efficiency of different insert fragments harboring various lengths of 3′-overlapping sequence in HARS36

Insert fragment	No. of transformants/μg of DNA
H625	33,670
H454	30,500
H150	31,920
H98	30,440
H39	6,820
T2	1,360
T1	1,730
L	1,400
Circular vector	1,989
Acceptor vector only	1,421