Figure 2.

(A) Northern analysis of VASA in fetal tissues (lanes a, testis; b, ovary; c, brain; d, thymus; e, lung; f, liver; g, spleen; h, pancreas; i, small intestine, colon, and stomach; j, kidney; k, adrenal, and l, placenta) and adult testis (lane m). The positions of size standards are indicated on the left. Approximately 5 μg of total RNA was loaded per lane. The blot was reprobed with a human β-actin probe to confirm RNA loading. (B) Northern analysis of VASA in adult tissues: lanes a, spleen; b, thymus; c, prostate; d, testis; e, ovary; f, small intestine; g, colon; and h, peripheral blood leukocytes. Each lane was loaded with 2.5 μg of poly(A)⁺ RNA from pooled specimens. The ovarian sample was prepared from ovaries of pre- and postmenopausal patients.