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. 2003 Aug;69(8):4628–4638. doi: 10.1128/AEM.69.8.4628-4638.2003

FIG. 7.

FIG. 7.

Tree-based representation of nine biochemically and genetically characterized RDases. The recently isolated PceA proteins from Dehalobacter restrictus (PceA-Dr) (EMBL accession number AJ439607) and Desulfitobacterium hafniense strain TCE1 (PceA-Dh-TCE1) (accession number AJ439608) are members of a very conserved group (group A) together with the PceA proteins of Desulfitobacterium hafniense strain PCE-S (PceA-Dh-PCE-S) (accession number AY216592) and Desulfitobacterium sp. strain Y51 (PceA-Dsp-Y51) (accession number AB070709). The group B RDases are chlorophenol RDases, including CprA of Desulfitobacterium chlororespirans (CprA-Dc) (accession number AF204275), CprA of Desulfitobacterium hafniense strain DCB-2 (CprA-Dh-DCB-2) (accession number AY013365), and CprA of Desulfitobacterium dehalogenans (CprA-Dd) (AF115542). TceA of Dehalococcoides ethenogenes (TceA-De) (accession number AF228507) and PceA of Dehalospirillum multivorans (PceA-Dm) (accession number AF022812) cannot be associated with any RDase group. The tree was constructed by using the neighbor-joining and bootstrapping tools of ClustalX and was rooted with the tetrachloro-p-hydroquinone RDase of Flavobacterium sp. (TeCH-Fl) (accession number PIR A40625).