TABLE 1.

Purification of PCE-RDase of Dehalobacter restrictus^a

Purification step	Sp act (U/mg of protein)b	Purification (fold)	Total activity (U)	Yield (%)
Crude extractc	0.61	1.0	1,818	100
Membrane fraction	0.82	1.3	1,443	79
Membrane extract	2.64	4.3	1,322	73
Mono Q	9.15	15.0	1,067	59
Superose 6	13.60	22.3	964	53

^aTCE-RDase activity always copurified with PCE-RDase activity.

^bOne unit of PCE-RDase activity was defined as oxidation of 2 μmol of methyl viologen/μmol of Cl⁻ formed/min at 30°C, as determined from initial rates of methyl viologen oxidation.

^c18.6 g (wet weight) of cells was used for purification.