TABLE 2.

Conversion of various nitriles by purified His-tagged nitrilase from Synechocystis sp. strain PCC6803

General compound group	Substrate	Relative activity (%)a
Benzene derivatives	Benzonitrile	100
	4-Aminobenzonitrile	28
	Benzylcyanide	52
	2-Chlorobenzylcyanide	6
	3-Chlorobenzylcyanide	316
	4-Chlorobenzylcyanide	11
	2-Methylbenzylcyanide	207
	4-Hydroxybenzylcyanide	10
2-3 Ring systems	2-Naphthalenecarbonitrile	757
	2-Naphthaleneacetonitrile	8
	9-Anthracenecarbonitrile	10
	3-Phenoxyphenylacetonitrile	35
Heterocyclic compounds	2-Thienylacetonitrile	311
	3-Thienylacetonitrile	102
	3-Cyanopyridine	245
	Indole-3-acetonitrile	354
Saturated aliphatic mononitriles	Propionitrile	33
	2-Chloropropionitrile	270
	3-Dimetylaminopropionitrile	8
	Butyronitrile	109
	Valeronitrile	222
	Isovaleronitrile	9
	2-Methylglutaronitrile	394
	Octanoic acid nitrile	303
	Dodecanoic acid nitrile	36
Saturated aliphatic dinitriles	Adipinic acid dinitrile	528
	Octanoic acid dinitrile	588
	Decanoic acid dinitrile	476
Unsaturated aliphatic mononitriles	Acrylonitrile	68
	2-Butenenitrile	49
	3-Butenenitrile	409
Unsaturated aliphatic dinitriles	Fumarodinitrile	12,000
	3-Hexenoic acid dinitrile	14

^aThe activity with benzonitrile was 100%. Less than 5% relative activity was found with the following substrates. 2-Ethyl-2-phenylbutyronitrile, 2-phenylvaleronitrile, 2,6-dichlorobenzylcyanide, naproxen nitrile, ketoprofen nitrile, acetonitrile, malononitrile, 2-methyl-3-butenenitrile, diethyldicyanofumarate, isobutyronitrile, 2-methylbutyronitrile, pentadecanenitrile, and nonadecanenitrile.