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Kinetic constants of nitrilase with different substrates
| Substrate | Vmax (U mg−1) | Km (mM) | Kcat (s−1) | kcat/Km (s−1 M−1) |
|---|---|---|---|---|
| Benzonitrile | 0.33 | 0.97 | 0.22 | 227 |
| Indole-3-acetonitrile | 1.5 | 2.4 | 1.0 | 417 |
| 2-Chloropropionitrile | 1.4 | 5.2 | 0.92 | 177 |
| Valeronitrile | 0.7 | 4.4 | 0.47 | 107 |
| Octanoic acid dinitrile | 0.7 | 0.2 | 0.47 | 2,350 |
| Decanoic acid dinitrile | 0.4 | 0.3 | 0.27 | 900 |
| Fumarodinitrile | 109 | 28 | 72.7 | 2,600 |
