Figure 7.

Time course of restoration of correct splicing and Hb A synthesis in mononuclear cells from patient ZA. Mononuclear cells on day 8 of culture were transfected with 45 μM 745–25 mer antisense oligonucleotide and incubated for 1–9 subsequent days with no additional oligonucleotide. Number of days after oligonucleotide treatment is indicated at the top (Upper); other designations as in Fig. 3 and 5. Lanes: 6–10, oligonucleotide-treated cells, lanes 1–5, no oligonucleotide controls. (Upper) Immunoblot of hemolysate of mononuclear cells probed with anti-human hemoglobin antibody. (Lower) Corresponding RT-PCR assay.