Skip to main content
. 2000 Aug 1;97(17):9597–9602. doi: 10.1073/pnas.150241797

Figure 4.

Figure 4

(A) cDNA panhandle PCR analysis of total RNA from t-AML of patient 36. Smear indicates products of heterogeneous sizes (lane Pt. 36). (B) PCR screen of representative recombination PCR-generated subclones of cDNA panhandle PCR products shown in A. MLL-containing subclones confirmed by sequencing are shown above respective lanes. (C) Summary of sequences in recombination PCR-generated subclones. Twelve subclones contained MLL sequence only (Upper). Incompletely processed and alternatively spliced transcripts and transcripts suggesting exon scrambling were detected. In three subclones, exon 5 sequence extended to position 3945 of MLL cDNA; the 3′ sequence was from exons 3, 4a, 4b, and 4c starting at position 2782 of MLL cDNA (GenBank accession nos. LO4284 and NM_005933). Positions 3945 and 2782 are the end and start of internal codons in MLL exons 5 and 3. Chimeric transcripts contained in-frame fusions of either MLL exon 7 or MLL exon 8 to position 979 of AF-10 cDNA (GenBank accession no. U13948) (Lower).