TABLE 2.
Effect of essential oils on growth of pure cultures of rumen bacteria after direct inoculation or after adaptation
| Strain | IC50 of essential oils (ppm)a
|
|
|---|---|---|
| Direct inoculation | Adaptation | |
| Clostridium sticklandii 12662 | 36.0 | 35.0 |
| Peptostreptococcus anaerobius 27337 | 42.5 | 52.0 |
| Selenomonas ruminantium Z108 | 57.0 | 58.0 |
| Ruminococcus flavefaciens FD1 | 60.0 | 60.0 |
| Prevotella brevis GA33 | 57.5 | 61.0 |
| Prevotella albensis M384 | 50.0 | 65.0 |
| Eubacterium ruminantium 2377 | 70.0 | 76.0 |
| Anaerovibrio lipolytica 5S | 73.8 | 80.0 |
| Veillonella parvula L59 | 88.0 | 86.0 |
| Prevotella ruminicola 23 | 33.8 | 94.3 |
| Fibrobacter succinogenes S85 | 95.0 | 110.0 |
| Butyrivibrio fibrisolvens SH13 | 56.2 | 112.5 |
| Prevotella bryantii B14 | 54.0 | 127.5 |
| Lachnospira multipara D15d | 112.5 | 120.0 |
| Ruminococcus albus SY3 | 49.0 | 132.5 |
| Ruminobacter amylophilus WP109 | 42.6 | 150.0 |
| Mitsuokella multiacidas 46/5 | 113.5 | 160.0 |
| Megasphaera elsdenii J1 | 113.0 | 190.0 |
| Lactobacillus casei LB17 | 56.2 | 221.2 |
| Streptococcus bovis ES1 | 127.5 | 240.0 |
| Clostridium aminophilum 49906 | 94.2 | 262.5 |
a
IC50 is the concentration of essential oils that led to a 50% decrease in cell density at 24 h of incubation. The results shown are means of three culture tubes at each concentration.