TABLE 1.
Metabolites of Candida species that were identified by 2D correlation NMR spectrad
| Compound | HSQC cross-peak | Metabolite concn (mM) for:
|
||||
|---|---|---|---|---|---|---|
| C. albicans | C. glabrata | C. krusei | C. parapsilosis | C. tropicalis | ||
| Lipidsa | 1.29/30.5 | 25-50 | 10-30 | 10-35 | 20-40 | 30-80 |
| Ala | 1.49/17.2 | 1-3 | 1-3 | 1-3 | 1-3 | 1-3 |
| Asp | 2.69/37.5 | 1-3 | 1-3 | 1-3 | <1 | <1 |
| Glu | 2.34/34.7 | 1-5 | 1-3 | 1-3 | 2-5 | 2-5 |
| Ile | 1.99/36.7 | ND | <1c | <1c | ND | ND |
| Leu | 1.7/40.6 | ND | <1c | ND | ND | ND |
| Lys | 3.02/40.5 | 1-10 | 1-5 | 3-10 | 1-5 | 2-8 |
| Val | 0.98/17.6 | ND | ND | <1c | ND | ND |
| Thr | 1.33/21.0 | ND | ND | <1 | ND | ND |
| Pheala | 3.29/37.7 | <1c | ND | <1 | <1 | <1c |
| Arg | 3.25/41.3 | ND | ND | ND | ND | <1 |
| Gaba | 3.04/39.9 | ND | ND | ND | ND | <1c |
| Trehalose | 5.19/94.0 | ND | 5-30 | 2-15b | Low | ND |
| Glucose | 5.23/92.9 | <1 | ND | ND | ND | <1 |
| Uridine | 7.87/142.6 | <1c | <1 | ND | ND | <1c |
| Ethanol | 3.65/58.3 | 15-40 | 10-40 | 10-30 | 5-25 | 5-15 |
| Acetate | 1.91/23.8 | 2-8 | 5-20 | 5-10 | <1 | <1 |
| Ribitol | 3.84/73.2 | Possibly | ND | ND | ND | ND |
| Glycerol | 3.78/72.3 | 5-15 | 2-10 | 5-15 | 5-10 | 8-20 |
| Arabitol | 3.90/71.0 | 5-10 | ND | 2-5b | 2-8 | 1-2c |
| Mannitol | 3.87/64.1 | ND | ND | <1 | ND | ND |
| Betaine | 3.27/54.1 | 1-3 | 1-3 | <1 | 1-3 | 1-3 |
| Dulcitol | 3.83/63.7 | ND | ND | ND | ND | 1-3 |
| GPC | 3.23/54.8 | <1c | 1-3 | ND | 2-5 | ND |
Lipid quantitation has not been corrected for the length of the fatty acid chain. The concentration refers to concentration of the represented CH2 units.
Of the five C. krusei isolates studied by {1H, 13C} HSQC spectra, one contained arabitol but no trehalose and the remaining four contained trehalose but no arabitol.
ND, not detected in all isolates.
The concentration of these compounds was determined relative to 10 mM p-aminobenzoic acid in the cell suspension and was based on the indicated {1H, 13C} HSQC cross-peak volumes, corrected for the representing number of carbon atoms. Concentration ranges were determined from at least three isolates per species. Organisms (5 × 108 to 2 × 109 CFU) were suspended in 0.5 ml of PBS/D2O to avoid sedimentation of cells in the NMR tube. Concentrations below 1 mM could not be determined reliably. The estimated error for concentration estimates of all other metabolites is of the order of 30 to 40% and confirms previous reports (4). Therefore, detectable cross-peaks of very low intensity were labeled as <1 mM.