TABLE 2.
Classification results for cultures that were misidentified at some stage of the classifier developmenta
| Stage | Isolate | Classification by VITEK/API | Classification by NMR/SCS | Classification by PCR fingerprinting after recultivation | Validation with final classifier |
|---|---|---|---|---|---|
| Development | WM 204 | C. albicans | Fuzzy (1) | C. albicans | C. albicans |
| of first classifiers | WM 923 | C. parapsilosis | Fuzzy (1) | C. parapsilosis | C. parapsilosis |
| (n = 162) | WM 1007 | C. parapsilosis | Fuzzy (1) | C. parapsilosis | C. parapsilosis |
| WM 929 | C. glabrata | C. krusei (2) | C. glabrata, atypical PCR | C. krusei | |
| Validation | WM 1029 | C. albicans | C. albicans (1), fuzzy (1) | C. dubliniensis | ND |
| of first classifiers | WM 1146 | C. glabrata | C. tropicalis (2) | C. tropicalis | C. tropicalis |
| (n = 145) | WM 1047 | C. glabrata | C. krusei (1) | Mixed, A, C. tropicalis; B, unidentified | A, C. tropicalis; B, ND |
| WM 1050 | Unidentified | C. krusei (2) | Unidentified | ND | |
| WM 1051 | Unidentified | C. albicans (1), C. glabrata (1) | Mixed, A, unidentified; B, Trichosporon beigelii | ND | |
| WM 1052 | Unidentified | C. glabrata (1), C. krusei (1) | Mixed, A, C. glabrata; B, unidentified | A, C. glabrata; B, ND | |
| WM 1070 | C. albicans | C. parapsilosis (1), fuzzy (1) | Mixed, A, C. parapsilosis; B, C. lusitaniae | A, C. parapsilosis; B, ND | |
| WM 1102 | C. glabrata | C. krusei (2) | C. glabrata | C. glabrata | |
| WM 1105 | C. krusei | C. parapsilosis (1) | C. krusei | C. krusei | |
| WM 1107 | C. glabrata | C. parapsilosis (1) | C. glabrata, atypical PCR | Fuzzy | |
| WM 1106 | C. glabrata | C. krusei (1), fuzzy (1) | C. krusei | C. krusei | |
| WM 1110 | C. parapsilosis | Fuzzy (1) | C. parapsilosis | C. parapsilosis | |
| WM 1119 | Filobasidiella neoformans | C. glabrata (2) | F. neoformans | ND | |
| WM 1151 | C. parapsilosis | C. glabrata (1) | C. parapsilosis | C. parapsilosis | |
| WM 1154 | C. krusei | Fuzzy (1) | C. krusei | C. krusei | |
| WM 1155 | C. parapsilosis | Fuzzy (1) | C. parapsilosis | C. parapsilosis | |
| WM 1156 | C. parapsilosis | C. glabrata (2) | C. parapsilosis | C. parapsilosis | |
| WM 1166 | C. parapsilosis | Fuzzy (1) | C. parapsilosis | C. parapsilosis | |
| WM 1170 | C. albicans | Fuzzy (1) | C. albicans | C. albicans | |
| WM 1223 | C. albicans | Fuzzy (1) | C. albicans | C. albicans | |
| Development | WM 921 | C. albicans | Fuzzy (1) | C. albicans | C. albicans |
| of second classifiers | WM 933 | C. albicans | C. tropicalis (1) | C. albicans | Fuzzy |
| (n = 355) | WM 1180 | C. albicans | Fuzzy (1) | C. albicans | C. albicans |
| ATCC 90030 | C. glabrata | Fuzzy (1) | C. glabrata | C. glabrata | |
| WM 1054 | C. krusei | C. parapsilosis (1) | C. krusei | C. glabrata | |
| WM 1006 | C. parapsilosis | C. tropicalis (1) | C. parapsilosis | C. parapsilosis | |
| WM 972 | C. parapsilosis | Fuzzy (1) | C. parapsilosis | C. parapsilosis | |
| WM 1007 | C. parapsilosis | C. albicans (1) | C. parapsilosis, type 3 | Fuzzy | |
| WM 1121 | C. glabrata | C. tropicalis (1) | C. glabrata | C. glabrata | |
| WM 1193 | C. albicans | C. tropicalis (1) | C. albicans | C. albicans | |
| WM 929 | C. glabrata | C. krusei (2) | C. glabrata, atypical PCR | C. krusei | |
| 1218666 | C. krusei | Fuzzy (1) | C. krusei | C. krusei | |
| Validation | WM 1210 | Y. lipolytica | C. parapsilosis (1), C. tropicalis | ND | ND |
| of second classifiers | WM 1207 | C. albicans | C. tropicalis (1), fuzzy (1) | C. albicans | Fuzzy |
| (n = 229) | WM 1191 | C. albicans | C. tropicalis (1) | C. albicans | C. albicans |
| WM 1173 | C. albicans | Fuzzy (1) | C. albicans | C. albicans | |
| 1059299 | C. glabrata | Fuzzy (1) | C. glabrata | C. glabrata | |
| JH23 | S. cerevisiae | C. glabrata (1), C. krusei (1) | ND | ND | |
| WM 01.177 | Unidentified | C. glabrata (1), fuzzy (1) | Unidentified | ND | |
| WM 01.176 | C. famata | C. parapsilosis (1), fuzzy (1) | C. parapsilosis | C. parapsilosis | |
| 7208673 | C. glabrata | Fuzzy (2) | C. glabrata | C. glabrata | |
| 7227274 | C. glabrata | Fuzzy (2) | C. glabrata | C. glabrata | |
| JH45 | S. cerevisiae | C. tropicalis (1), C. glabrata (1) | ND | ND | |
| WM 1124 | S. cerevisiae | C. glabrata (2) | ND | ND | |
| WM 1227 | S. cerevisiae | C. glabrata (2) | ND | ND | |
| WM 01.179 | Unidentified | C. krusei (1), fuzzy (1) | Kluyveromyces marxianus var. marxianus | ND | |
| WM 01.180 | C. zeylanoides | C. glabrata (1), C. krusei (1) | Unidentified | ND | |
| WM 01.181 | C. zeylanoides | C. krusei (1), fuzzy (1) | C. glabrata, atypical PCR | Fuzzy | |
| WM 258 | Unidentified | C. tropicalis (1), fuzzy (1) | C. nitrativorans | ND | |
| WM 599 | Y. lipolytica | C. tropicalis (2) | Y. lipolytica | ND | |
| WM 01.14 | Unidentified | C. parapsilosis (1), fuzzy (1) | C. parapsilosis | C. parapsilosis/PICK> | |
| WM 1070 | Unidentified | C. tropicalis (1), fuzzy (1) | Mixed, A, C. parapsilosis; B, C. tropicalis | A, C. parapsilosis; B, C. tropicalis | |
| WM 1009 | F. neoformans | C. glabrata (1), fuzzy (1) | F. neoformans | ND | |
| WM 1124 | S. cerevisiae | C. glabrata (2) | S. cerevisiae | ND | |
| WM 01.195 | C. tropicalis | Fuzzy (1) | Mixed, A, C. tropicalis; B, unidentified | A, C. tropicalis; B, ND | |
| WM 01.199 | C. krusei | C. krusei (1), C. tropicalis (1) | Unidentified | ND | |
| WM 01.211 | ND | C. tropicalis (2) | Y. lipolytica | ND | |
| WM 01.205 | C. tropicalis | C. albicans (1), C. tropicalis (1) | Mixed, A, Y. lipolytica; B, unidentified | ND | |
| Development | WM 221 | C. albicans | Fuzzy | C. albicans | |
| of final classifiers | WM 1207 | C. albicans | Fuzzy | C. albicans | |
| (n = 444) | WM 02.03 | C. albicans | Fuzzy | C. albicans | |
| WM 929 | C. glabrata | Fuzzy | C. glabrata, atypical PCR | ||
| WM 01.181 | C. glabrata | Fuzzy | C. glabrata, atypical PCR | ||
| WM 1142 | C. glabrata | Fuzzy | C. glabrata | ||
| WM 1146 | C. glabrata | Fuzzy | C. glabrata | ||
| WM 932 | C. krusei | Fuzzy | C. krusei | ||
| WM 990 | C. krusei | C. glabrata | C. krusei | ||
| WM 993 | C. krusei | Fuzzy | C. krusei | ||
| WM 1154 | C. krusei | Fuzzy | C. krusei | ||
| 00-027-1760 | C. krusei | C. parapsilosis | C. krusei | ||
| 99-237-2930 | C. krusei | Fuzzy | C. krusei | ||
| WM 1003 | C. parapsilosis | Fuzzy | C. parapsilosis | ||
| WM 1007 | C. parapsilosis | Fuzzy | C. parapsilosis, type 3 | ||
| WM 01.18 | C. parapsilosis | Fuzzy | C. parapsilosis, type 3 | ||
| WM 01.56 | C. parapsilosis | Fuzzy | C. parapsilosis, type 3 | ||
| WM 930 | C. tropicalis | C. albicans | C. tropicalis | ||
| WM 981 | C. tropicalis | C. parapsilosis | C. tropicalis | ||
| Validation | WM 02.57 | C. glabrata | C. tropicalis (1) | C. glabrata | |
| of final classifiers | WM 02.05 | C. albicans | C. parapsilosis (1) | C. albicans | |
| (n = 133) | WM 02.78 | C. krusei | C. tropicalis (1) | C. krusei | |
| WM 01.203 | C. tropicalis | C. glabrata (1) | C. tropicalis | ||
| WM 02.08 | C. krusei | Fuzzy (1) | C. krusei | ||
| WM 02.86 | C. tropicalis | Fuzzy (1) | C. tropicalis |
a
The number in parentheses indicates the number of misidentified cultures (all isolates were cultured as duplicates except for the development and test of the final classifiers. All isolates were recultured and tested against the final classifiers except for isolates that were identified as species that were not part of this study. n indicates the total number of cultures in the respective data set. In cases of mixed cultures, the purified cultures were PCR fingerprinted. Purified cultures were labeled as A, B,.... “Unidentified” refers to indeterminate identification by VITEK and API tests and by PCR fingerprinting when the respective pattern did not match with one of the five studied Candida species or species studied by Latouche et al. (23). The comment “mixed” refers to the fact that no pure culture was obtained after recultivation of the isolate from storage. The purified isolates are named A and B. ND indicates that the respective test was not done.