Figure 2.

Pedigree Lund 5 shown with the CGH profiles of chromosome 13, demonstrating loss of 13q21-q22 in all five tumors from the four affected individuals. The haplotypes with markers from 13q are shown next to the CGH profiles (the marker map is located on the left). Markers located near BRCA2 were not shared between the affected individuals (D13S260 and D13S267). At 13q21-q22, however, all five affected women shared a 7 cM haplotype (red box). Below the CGH profile of a bilateral breast cancer is the original CGH image (FITC hybridization with tumor DNA). The interstitial 13q21-q22 loss can be observed as an absence of FITC signal (green color) in the middle of the chromosome (a). Hybridization with a Texas red-labeled yeast artificial chromosome probe for marker D13S1257 demonstrates that the region of the shared haplotype coincided with the region of deletion by CGH (b).