TABLE 2.

Incidence of seed contamination using different inoculation methods and soils^a

Sample	No. of contaminated seed pools (no. tested)
	Autoclaved soil		Unautoclaved soil		Amended soil
	S. enterica	E. coli O157:H7	S. enterica	E. coli O157:H7	S. enterica	E. coli O157:H7
Inoculated seedlings	18 (143)	34 (127)	3 (9)	3 (9)	1 (9)	4 (9)
Coinoculated seedlings	0 (18)	0 (18)	NDb	ND	ND	ND
Inoculated seed	0 (9)	0 (9)	0 (9)	0 (9)	0 (9)	0 (9)
Inoculated soil	1 (9)	0 (9)	0 (9)	1 (9)	1 (9)	0 (9)

^aIncidence of contamination in 1,000-seed samples from each seed pool. The number in parentheses is the number of seed pools tested. Seedlings were growing sterilely (gnotobiotic) when inoculated and transferred to soil after 10 days or coinoculated with E. asburiae and one of the indicated pathogens.

^bND, not determined.