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Effect of temperature on chromate reductase activity. The basic enzyme reaction mixtures containing GST-EcNfsA (▪), GST-EcNfsB (□), or GST-VhNfsA (•) were incubated at 4 to 80°C, and their chromate-reducing activities were determined. The chromate reductase activity of GST-EcNfsA at 55°C, GST-EcNfsB at 30°C, or GST-VhNfsA at 30°C was regarded as the 100% value.
