FIG. 2.
Partial restriction map of the DNA fragment containing the steroid degradation genes of C. testosteroni TA441. Large open arrows, genes and putative ORFs; small open arrowheads above large arrows, putative promoters. Putative terminators are located just downstream of tesG and ORF18. Small solid arrowheads above genes and ORFs indicate restriction sites where the Kmr gene was inserted. DNA segments used as probes are shown just below the ORFs. Deletion plasmids and remaining gene segments are also shown below the restriction map. Abbreviations: Bm, BamHI; Bg, BglII; EI, EcoRI; EV, EcoRV; H, HindIII; K, KpnI; Mn, MunI; Nr, NruI; Nt, NotI; Ps, PstI; Sc, SacI; Sl, SalI; Sm, SmaI; Sn, SnaBI; Xb, XbaI; Xh, XhoI.