FIG. 6.
Growth of TA441 and gene disruption mutants on testosterone (a) or androsterone (b) as the sole carbon source. The genes were disrupted by insertion of a Kmr gene. Each strain was grown in LB medium, washed twice, and inoculated into 10 ml of C medium supplemented with 0.1% (wt/vol) testosterone or androsterone. Symbols: ▪, TA441; ▴, TesH disruption mutant; ▵, TesI disruption mutant; •, ORF17 disruption mutant; ○, ORF18 disruption mutant. Growth is expressed in CFU. Data are averages of more than three experimental determinations.