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. 2003 Aug;69(8):4534–4541. doi: 10.1128/AEM.69.8.4534-4541.2003

TABLE 4.

Km and Vmax valuesa determined for phenylpyruvate decarboxylase activity in cell extracts from three S. cerevisiae strains grown in aerobic glucose-limited chemostats with phenylalanine as the sole nitrogen source (dilution rate, 0.1 h−1)

Strain Relevant genotype Km (mM) Vmax (nmol mg of protein−1 min−1)
CEN.PK 113-7D PDC1 PDC5 PDC6 YDR380w YDL080c 0.10 ± 0.001 54 ± 6
CEN.PK 608-4B pdc1 pdc5 pdc6 YDR380w ydl080c 0.062 ± 0.005 38 ± 3
CEN.PK 555-4A PDC1 PDC5 PDC6 ydr380w YDL080c 0.48 ± 0.08 240 ± 28
a

The values are means ± maximal deviations derived from two independent experiments for CEN.PK 113-7D and CEN.PK 608-4B and from three independent experiments for CEN.PK 555-4A.