FIG. 2.
Representative SYBR Green I amplicon melt curves analyzed with LightCycler Data Analysis software, version 3.1.1.06i, with the digital filter enabled and polynomial curve fitting. Shown are results for the no-template control (— - —), the distantly related strain B. bronchiseptica ATCC 10580 (------), B. abortus NADC 1144 (——), B. abortus biovar 1 ATCC 23448 (coincident with NADC 1144) (——), B. abortus biovar 3 ATCC 17385 (doublet peaks with markedly higher Tm) (——), O. anthropi (— .. —), R. leguminosarum (— — —), and R. rhizogenes ATCC 15834 ( ).