TABLE 1.
Specificity of real-time PCR assays for the detection of B. abortus
| Template origin | Strain | Source | Result by:
|
||
|---|---|---|---|---|---|
| SYBR Green I assay | 5′-Exonuclease assay | Hybridization probe assay | |||
| Brucella abortus (standard curve reference strain)a | NADC 1144 | YNP | + | + | + |
| B. abortus | ATCC 4315 | YNP | + | + | + |
| B. abortus bv. 1 | ATCC 23448 | YNP | + | + | + |
| B. abortus bv. 2 | ATCC 23449 | YNP | + | + | + |
| B. abortus bv. 3 | ATCC 17385 | YNP | + | + | + |
| B. abortus bv. 4 | ATCC 23451 | YNP | + | + | + |
| B. abortus bv. 5 | ATCC 23452 | YNP | + | + | + |
| B. abortus bv. 9 | ATCC 23455 | YNP | + | + | + |
| B. abortusa | NADC 1145 | YNP | + | + | + |
| B. abortusa | NADC 1403 | YNP | + | + | + |
| B. abortusa | NADC 1409 | YNP | + | + | + |
| B. abortus | NADC 2308 | YNP | + | + | + |
| B. abortus | NADC RB51 | YNP | + | + | + |
| B. canis | ATCC 23365 | AFIP | + | + | ± |
| B. canis | ATCC 6133 | AFIP | + | + | − |
| B. melitensis | ATCC 16M | AFIP | + | − | − |
| B. neotomae | ATCC 23459 | AFIP | + | − | − |
| B. ovis | ATCC 25840 | AFIP | + | − | − |
| B. suis | ATCC 23444 | AFIP | + | − | − |
| Bordetella bronchiseptica | ATCC 10580 | ATCC | + | − | − |
| Ochrobactrum anthropi | ATCC 49237 | ATCC | + | − | − |
| Rhizobium leguminosarum | ATCC 14479 | ATCC | + | − | − |
| R. radiobacter | ATCC 15955 | ATCC | + | − | − |
| R. radiobacterb | ATCC 19358 | ATCC | + | − | − |
| R. radiobacterb | Field isolate | Our lab | + | − | − |
| R. rhizogenes | ATCC 15834 | ATCC | + | − | − |
| Sinorhizobium meliloti | ATCC 10310 | ATCC | + | − | − |
a
Bison isolate.
b
Strains previously classified as Agrobacterium tumefaciens.