TABLE 4.
Melt curve analysis of SYBR Green I amplification products
| Template origin | Avg amplicon Tm (°C)a |
|---|---|
| B. abortus NADC 1144, 1145, 1403, 1409, 2308, RB51, bv. 1, 2, 4 | 86.4 |
| B. abortus bv. 3, 5, 9 | 85.8, 89.5 |
| B. canis, B. melitensis, B. neotomae, B. ovis, B. suis | 89.6 |
| R. rhizogenes ATCC 15955, R. rhizogenes (field isolate), S. meliloti ATCC 10310, O. anthropi ATCC 49237 | 89.2 |
| R. radiobacter ATCC 19358, R. rhizogenes ATCC 15834 | 85.6 |
| R. leguminosarum ATCC 14479 | 91.6 |
| B. bronchiseptica ATCC 10580 | 80.5 |
a
Tms listed are for each prominent peak in the melt curve analysis.