TABLE 3.
Optimized PCR protocol from each species tested
| Species | Primer pair | Cycles | Annealing temp (°C) | Size of amplified product (bp) |
|---|---|---|---|---|
| L. curvatus | Lc-16S | 20 | 50 | 305 |
| L. plantarum | Lpl-16S | 30 | 50 | 265 |
| L. sakei | Ls-16S | 20 | 45 | 290 |
| E. faecium | Ef-16S | 20 | 55 | 676/578 |
| L. lactis | Lac-23S | 20 | 59 | 2,060 |
| P. acidilactici | Pda-23S | 20 | 55 | 2,023/1,767 |
| S. carnosus | Sca1-Sca2 | 35 | 67 | 305 |
| K. varians | Kva-Kva2 | 35 | 67 | 3,362 |
| S. warneri | Swa-Sta2 | 20 | 72 | 289 |
| S. xylosus | Fa/Fb-Ra/Rb | 35 | 58 | 417/317/217 |
| S. epidermidis | Epi1-Epi2 | 30 | 55 | 240 |
| S. simulans | Sim1-Sim2 | 30 | 55 | 220 |