Figure 6.

In vivo angiogenic activity of Smad4/DPC4-reconstituted clones and negative control. (A) Hydron pellets containing conditioned medium (200 μg/ml) alone or with inducer (1 ng/ml VEGF or 100 ng/ml basic fibroblast growth factor) or neutralizing antibody to VEGF (5 μg/ml) or TSP-1 (antibody A4.1 at 20 μg/ml) were implanted into the avascular rat cornea. Vigorous in-growth of vessels from the limbus toward the pellet by 7 days was scored as a positive response. (B) Representative photos of corneal responses from A. (Bars = 200 μm.)