TABLE 2.
Eimeria species that may be amplified with primers CYCAI2 and CYCAR1
| Organism (reference) | Accession no. | Host | Distribution | Inner forward primer (CYCAI2)a | Tm (°C)b | Amplicon size (bp) | Alu I fragment sizes (bp) |
|---|---|---|---|---|---|---|---|
| E. acervulina (42) | U67115 | Chicken | Worldwide | CGGGGCTGGGTAATCTTCTGAG | 69.4 | 254 | 156, 98 |
| E. bovis (23) | U77084 | Cattle | Worldwide | GAAGTCTAGGTAATCTTTTGAG | 52.4 | 253 | 156, 97 |
| E. brunetti (24) | U67116 | Chicken | Worldwide | CGGGGCTGGGTAATCTTGTGGG | 73.7 | 253 | 155, 98 |
| E. falciformis (35) | AF080614 | Mus (mouse) | NA,c Europe | CAGGTCTAGGTAATCTTTTGAG | 55.5 | 255 | 159, 96 |
| E. meleagrimitis (41) | AF041437 | Meleagris (turkey) | United States | GCAGTCTAGGTAATCTTTTGAG | 55.7 | 254 | 159, 95 |
| E. mitis (42) | U67118 | Chicken | Worldwide | TGGGCGTGGGTAATCTTGTGAG | 69.7 | 250 | 157, 94 |
| E. mivati (12) | U76748 | Chicken | NA, Europe | CGGGGCTGGGTAATCTTGTGAG | 70.2 | 254 | 158, 96 |
| E. nieschulzi (7) | U40263 | Rattus (rat) | Worldwide | CAGGTCTAGGTAATCTTTTGAG | 55.5 | 255 | 157, 98 |
| E. praecox (22) | U67120 | Chicken | Worldwide | TGGGGCTGGGTAATCTTGTGAG | 68.8 | 252 | 158, 94 |
a
Underlined letters are changes in sequence from CYCAI2.
b
Calculated as described by Rychlik and Rhoads (34).
c
NA, North America (includes Canada, the United States, and Mexico).