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Purification of mutated PhaJAc (V130G) from recombinant E. coli BL21(DE3)
| Step | Total activitya (U) | Total protein (mg) | Sp acta (U/mg) | Purifi- cation (fold) | Recovery (%) |
|---|---|---|---|---|---|
| None | 3.9 × 103 | 62 | 62 | 1 | 100 |
| (NH4)2SO4 precipita- tion (40-60% cut) | 2.8 × 103 | 36 | 78 | 1.3 | 73 |
| Phenyl Sepharose | 1.5 × 103 | 4.0 | 373 | 6.0 | 38 |
| MonoQ | 1.1 × 103 | 1.2 | 883 | 14 | 28 |
Enoyl-CoA hydratase activity toward crotonyl-CoA.
