Table 2.

Horizontal spread and systemic infection of the P. aeruginosa ppk mutant in mouse tissues

Strain	CFU/g tissue*
	Horizontal spread within skin				Systemic infection
	8 h, Inoc.	8 h, Dist.	24 h, Inoc.	24 h, Dist.	Spleen	Liver
	×104	×104	×107	×107	×103	×102
PAO1 (WT)	180  ± 70	30  ± 7.6	180  ± 32	120  ± 28	460  ± 65	309  ± 61
PAOM5 (ppk)	4.4  ± 1.8	0	120  ± 57	39  ± 10	2  ± 1	1  ± 0.4

^*Mice were injected with ≈200 cells. CFUs were measured at 8 h and 24 h post-burn infection for horizontal spread within the skin and at 24 h for systemic infection in spleen and liver. Values represent averages ± SEM for 15 measurements in three separate experiments. Inoc., inoculation site; Dist., distant site (1.5 cm away).