Figure 5.
Early vacuoles contain cytochrome c but the late ones do not. Spinal cord sections from SOD1G93A mice at the pre-muscle-weakness (PMW) stage were stained with anti-SOD1 (red) and anti-cyt c (green) antibodies (A-H), or anti-SOD1 (red) and anti-CO1 (green) antibodies (I-L), and shown as 3-D reconstructed confocal images. C, G and K are superimpositions of the red and green signals. D, H and L are the same as C, G and K, respectively, but rotated to the right for 30°. Arrows point to small vacuoles and arrowheads point to SOD1 aggregates. Early vacuoles contain cytochrome c (E-H) but the late ones do not (A-D). Notice that the inner member marker CO1 does not colocalize with SOD1 (I-L).