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. 2000 Aug 1;97(17):9648–9653. doi: 10.1073/pnas.160469097

Figure 2.

Figure 2

PfRBP2-Ha and -Hb share >8 kb of common sequence. (A) PfRBP2-Ha (1.1 and 08813) and -Hb (18.2 and 04278) schematic. Gray, black, and hatched boxes define shared, repeat, and unique regions, respectively. Primers used in long-range PCR are marked (SF7, SF5, SF3, AR2, and BR2), as are EcoRV (RV) and HindIII (H) restriction sites and fragments used as probes in Southern blots (Sh1, Sh2, A, and B). (B) Long-range PCR performed on FVO DNA by using reverse primers from the unique regions (AR2 or BR2) paired with forward primers designed from contig 04278 (SF3, SF5, and SF7). (C) Southern blots of EcoRV (RV) and HindIII (H) -digested FVO DNA, probed with fragments Sh1, A, B, and Sh2. DNA 1-kb ladder markers from GIBCO/BRL are indicated.