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LPS induction of IRF-1. The dosage-dependent LPS activation of IRF-1 transcription was measured in serum-free (A) and serum-supplemented (B) A549 cell cultures. Relative levels of IRF-1 transcripts were measured by RT-PCR as described in the legend to Fig. 4. The IRF-1 mRNA intensity of each signal from A549 cells exposed to various LPS concentrations were normalized to the 18S rRNA standard. The data from the time-course experiment (Fig. 4A) are included for comparison.
