Table 1.
Cell death and proliferation in sympathetic explants
Treatment | BrdUrd | TUNEL |
---|---|---|
NGF → NGF | 24.2 ± 11.3 (n = 30) | 9.0 ± 7.9 (n = 56) |
NGF → NT3 | 30.5 ± 10.6 (n = 42) | 15.8 ± 6.8 (n = 56) |
NT3 → NT3 | 26.3 ± 7.2 (n = 36) | 30.4 ± 16.8 (n = 19) |
NT3 → NGF | 26.6 ± 5.6 (n = 51) | 22.5 ± 15.4 (n = 36) |
E12 ganglia were cultured for 4 days in the presence of NGF or NT3 (10 ng/ml). Subsequently the same factor was added again or was replaced by the other. Twenty-four hours later explants were fixed and the number of TUNEL-positive or BrdUrd-positive nuclei per ganglion was determined.