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. 2000 Aug 8;97(17):9689–9694. doi: 10.1073/pnas.160249097

Figure 1.

Figure 1

Construction and expression of a transgenic vector encoding caveolin-3. (A) The caveolin-3 transgenic expression cassette. The full-length untagged cDNA encoding caveolin-3 was subcloned into the expression vector pCAGGS. Note that in the pCAGGS-Cav-3 construct, the CMV enhancer and the chicken β-actin promoter sequence are located upstream the caveolin-3 cDNA, whereas the rabbit β-globin poly(A) sequence is located downstream of the caveolin-3 cDNA. The CMV enhancer/β-actin promoter sequences are expected to drive constitutive expression of caveolin-3 in a wide variety of tissue types. (B) Western blot analysis. The expression of caveolin-3 in normal control mice (CTL) and caveolin-3 transgenic mice (CAV-3) is shown. Protein lysates were prepared from a variety of mouse tissues. Immunoblotting was performed with a mono-specific antibody probe that recognizes only caveolin-3 (mAb 26). Note that in normal control mice caveolin-3 is expressed only in heart and skeletal muscle. In contrast, caveolin-3 transgenic mice express caveolin-3 in all tissues examined and demonstrate elevated levels of caveolin-3 in heart and skeletal muscle. Each lane contains an equal amount of total protein.