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. 2000 Aug 8;97(17):9712–9717. doi: 10.1073/pnas.160115697

Figure 2.

Figure 2

BACE2 cleaves APP(662–679) at the β site. HEK293T cells were transfected with vector alone, C-terminally tagged BACE1, or C-terminally tagged BACE2 as indicated, lysed, and precipitated with 1D4-Sepharose beads. Beads were washed and incubated with 50 μM APP(662–671) peptide and analyzed by TOF-MALDI. ++, Double protonation of the indicated peptide fragment; and *, N-terminally truncated peptide fragments observed in untreated peptide. Peaks at 1599.6 Da (BACE1) and 1606.5 and 1840.1 Da (BACE2) are not assignable to any peptide fragment. Peptide fragments of 976.4 and 1193.6 correspond to N- and C-terminal fragments of the peptide cleaved at the β site. The peptide sequence with APP and Aβ numbering is displayed below the figures.