Figure 2.

Dilator responses to endothelium-dependent and -independent agonists in the isolated perfused hindlimb of WT mice. (A) Original tracing showing the effect of bolus applications (100 μl) of ACh or SNP on perfusion pressure in the absence or presence of l-NA (300 μmol/liter). Numbers above the arrows indicate the dose of the agonist applied with the bolus (log mol); ∗ indicates a reduction in flow rate to maintain the perfusion pressure at a constant level. (B) Parameters of the perfused hindlimb in WT mice under basal conditions (shaded columns), during application of l-NA (filled columns), and after reduction of flow rate to adjust perfusion pressure during l-NA application (open columns). (C) Vasodilator responses to bolus applications (100 μl) of ACh and SNP in WT mice under control conditions (□), during the infusion of l-NA (■), and during infusion of l-NA and flow rate adjustment (●); n = 8 each group; ∗, P < 0.05. All experiments were performed in the presence of diclofenac (10 μmol/liter).