Fig. 1.

Enzymatic activity of ZmCKX1 peptide constructs and induction of zoospore encystment. (A) Enzymatic activity (AU) of constructs secreted from Pi. pastoris. Secreted ZmCKX1 peptides were concentrated and purified before activity measurement. (B) Premature encystment of Ph. capsici zoospores induced by ZmCKX1 peptides. A 20-μl droplet containing ≈500 zoospores was incubated with Pi. pastoris-expressed scaffold peptides (final concentration of 0.5 μg/ul) for 90 min at room temperature. The number of zoospores encysted was counted and expressed as a percentage. The error bars indicate standard deviations (n = 6).