Table 1.
Construct | Sense primer* | Antisense primer |
---|---|---|
Actin† | ACCCAATTGAGCACGGAATT | GCCAATGCAGTAATTTCTTTGC |
wt‡ | CACGGCCCACAGATATCTAAT | CTAGACTAGTCAAAGCTTGTTG |
AKK 1467B | Same | Same |
CKX | Same | Same |
CKX-Pc42 | Same | TCCATCATGTACATTAGGTGATAC |
CKX-Pc87 | Same | TGTTGGTGACATTGATGGTCTATC |
CKX-His | Same | TCCGTGGTGGTGGTGGTGGTG |
*All primer sequences are oriented 5′ to 3′.
†The tomato actin gene (GenBank accession no. U60480) was used as an internal reference.
‡Nontransformed roots.