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. 2006 Oct 9;103(49):18444–18449. doi: 10.1073/pnas.0605542103

Table 1.

Primers for detection of ZmCKX1 peptide expression in tomato hairy roots

Construct Sense primer* Antisense primer
Actin ACCCAATTGAGCACGGAATT GCCAATGCAGTAATTTCTTTGC
wt CACGGCCCACAGATATCTAAT CTAGACTAGTCAAAGCTTGTTG
AKK 1467B Same Same
CKX Same Same
CKX-Pc42 Same TCCATCATGTACATTAGGTGATAC
CKX-Pc87 Same TGTTGGTGACATTGATGGTCTATC
CKX-His Same TCCGTGGTGGTGGTGGTGGTG

*All primer sequences are oriented 5′ to 3′.

The tomato actin gene (GenBank accession no. U60480) was used as an internal reference.

Nontransformed roots.