Fig. 1.

Accumulation of NHEJ proteins on laser-induced damage. (A) Accumulation of Ku80, EGFP-Ku80, γ-H2AX, and TUNEL staining in V79B cells after laser irradiation. The leftmost panels show the position of the laser-induced damage as dotted gray lines. (B) EGFP-Ku80 accumulation intensities correlate with DNA-dense heterochromatic regions. The chromatin density is revealed by the DNA dye DRAQ5 introduced after laser irradiation was performed. The image was taken 30 min after irradiation. (C) Accessibility of condensed chromosomes to DNA end binding of EGFP-Ku80. The image was taken 10 min after irradiation. (D) A representative cell division of EGFP-Ku80-expressing XR-V15B cells. The time in hours after irradiation is given in each frame. (Scale bars: 5 μm.)