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Accumulation of NHEJ proteins on laser-induced damage. The immunofluorescent staining was done with antibodies to T2609 phosphorylated DNA-PKCS in HeLa cells or XRCC4 in V79B hamster cells. EGFP-ligase IV was directly imaged in primary human fibroblasts derived from a SCID patient with a ligase IV mutation that renders the endogenous protein unstable. (Scale bars: 5 μm.)
